Epitope analysis of a novel Homo spains synapse associated protein(FRG4) and preparation of antibody
- VernacularTitle:新的人突触相关蛋白(FRG4)抗原表位分析及抗体制备
- Author:
Ren WANG
;
Xiangdong YANG
;
Shunlin QU
;
Junwen LIU
;
Hongwei LV
;
Zhijuan JI
;
Shuli SHENG
- Publication Type:Journal Article
- Keywords:
Macrophages;
Atherosclerosis;
Synapse associated protein;
Peptide synthesis;
Antibody
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze a novel epitope of Homo sapiens synapse associated protein and synthesize polyclonal antibody.Methods:FRG4 full-length sequence was obtained by PCR from human fetal liver library;by bioinformatics to detect the second structure of amino acids encoded by FRG4 and its epitope and motifs;by solid-phase peptide synthesis method to synthesize FRG4 peptides,then peptides were immunized to rabbits;by immunohistory to detect the expression of FRG4 in HepG_2 cells.Results:Select 13-peptides PKLVKEEVFWRNY by bioinformatics to synthesize rabbit anti-human FRG4 polyclonal antibody.Antibody purity was 82.79% and antibody dilution was 1∶16 000 detected by ELISA.The antibody had a good reaction and speciality in Western blot,it was mainly expressed in cytoplasm of HepG_2 cells.Conclusion:A novel Homo sapiens synapse associated protein(FRG4) antibody was synthesized successfully.
