Effect of intracoronary adenoviral mediated gene transfer of transforming growth factor ?_1 on acute vas-cular rejection of discordant cardiac xenograft
- VernacularTitle:转染转化生长因子?_1基因减轻非协调性异种心脏移植急性血管性排斥反应
- Author:
Xueshan HUANG
;
Daozhong CHEN
;
Liangwan CHEN
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Transplantation,heterologous;
Transforming growth factor beta;
Gene transfer;
Graft rejection
- From:
Chinese Journal of Organ Transplantation
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of intracoronary adenovirus vector-mediated transforming growth factor ?_1 (Ad.TGF-?_1) gene transfer on discordant cardiac xenograft in the setting of acute vascular rejection. Methods In a cervical heterotopic cardiac transplantation model by cuff technique, after harvest, guinea pig donor hearts’coronary arteries were perfused ex vivo with Stanford University solution containing 5?1010 plaque-forming units/g of donor heart of Ad.TGF-?_1, then implanted in the necks of complement depleted and immunosuppressed rat recipients. As controls, other hearts were perfused with Stanford University solution containing 5?1010 plaque-forming units/g of donor heart adenoviral blank-vector or with virus-free Stanford University solution by the same method.Results The exogenous TGF-?_1 gene transcripts and expression in the Ad.TGF-?_1 infected grafts were confirmed. The number of inflammatory cells and macrophages and nature killer cell infiltration in the cardiac xenografts of Ad.TGF-?_1 infected grafts was less than that of other groups (P
