Transplantation of adrenocortical cells using poly-?-hybroxybutyric acid as a cell carrier
- VernacularTitle:以聚?-羟基丁酸酯为支架的肾上腺细胞移植形态学研究
- Author:
Tianpeng WU
;
Ping HU
;
Xiaobin ZHANG
- Publication Type:Journal Article
- Keywords:
Adrenal gland;
Cell transplantation;
Poly-?-hybroxybutyric acid;
Biopsy
- From:
Chinese Journal of Organ Transplantation
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the morphology change of transplants of adrenocortical cells using poly-? -hybroxybutyric acid (PHB) as a cell carrier. Methods Adrenocortical cells were seeded on PHB and then implanted into rats bearing bilateral adrenalectomy. Histological changes and CD3 expression by using immunohistochemical staining were observed in local area of transplants on the postoperative week 2, 4, 8. Results Histological study showed inflaming cells were decreased as time passed, fibrous tissue disappeared step by step, PHB degraded gradually and adrenocortical cells tended to be organized; Ultrastratural observation revealed these cells maintained identical secreting function; Immunohistochemical assay indicated the expression of CD3 in the transplanted was similar to that in the normal adrenal cells. Conclusion The transplanted cells seeded in PHB can not only be survival, but also organized to the same tissue structure and ultrastructure as normal adrenal tissue.
