Establishment of the method for determination of serum citrulline and its application in intestinal transplantation
- VernacularTitle:血清瓜氨酸测定方法的建立及在小肠移植中的应用
- Author:
Fangnan LIU
;
Li TAN
;
Nan LUO
- Publication Type:Journal Article
- Keywords:
Transplantation;
Intestine,small;
Citrulline;
Chromatography,high pressure liquid
- From:
Chinese Journal of Organ Transplantation
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the level of serum citrulline and its significance in the patients subject to intestinal transplantation. Methods Phenylisothiocyanate served as derivative reagent and the serum citrulline was determined by HPLC. Thirty-three healthy adults served as controls. Serum citrulline was measured in 2 cases of intestinal transplantation during different transplant periods. Results The serum citrulline level in 33 healthy adults was (16.87?5.97)?mol/L. Before operation, it was low (less than 10 ?mol/L) in the patients and after operation it reached to high levels and kept at higher levels. The maximium of serum citrulline was more than 30 ?mol/L in patient A and was over 50 ?mol/L in patient B. Following rejection, the serum citrulline level in patient A was decreased rapidly and simialr to that before operation and in patient B it was lower than that before operation. Conclusion The level of serum citrulline in intestinal transplantation patients was correlated with transplant rejection. More patients are reqiured for the further study.
