Effect of 2?-(3-hydroxypropoxy)-calcitriol on proliferation and differentiation of osteoblast-like cells
- VernacularTitle:2?-(3羟丙氧)-骨化三醇对OS-732人成骨样细胞增殖与分化的影响
- Author:
Yanlan YANG
;
Jie LIU
;
Yan XUE
- Publication Type:Journal Article
- Keywords:
ED-71;
Cell differentiation;
Osteoblasts;
Calcitriol
- From:
Chinese Journal of Geriatrics
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of 2?-(3-hydroxypropoxy)-Calcitriol (ED-71) on the cell growth and differentiation of human osteoblast-like cell line OS-732. Methods Osteoblast-like cells were cultured with various concentration of ED-71.The effects of ED-71 on proliferation and differentiation were detected by (MTT), alkaline phosphatase activity and level of osteocalcin (bone gla protein,BGP). Results The proliferation rate of osteoblast-like cells cultured for 4 days was significantly inhibited by 10~ -7mol/L concentration of ED-71. ED-71 in 10~ -8, 10~ -9mol/L concentration can significantly increase BGP level, but ED-71 in 10~ -7mol/L concentration may decrease BGP level.Alkaline phosphatase activity compared with control was not changed significantly. Conclusions ED-71 can inhibit cell growth in high concentration and improve cell differentiation in lower concentration.
