Semi-quantitative reverse transcription-PCR assay for renal cell carcinoma-associated protein G250 mRNA
- VernacularTitle:肾癌相关蛋白G250 mRNA在肾细胞癌中表达的研究
- Author:
Haijiao CHEN
;
Chuanzhong YE
;
Li ZHANG
;
Guomin WANG
;
Yongkang ZHANG
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
G250
- From:
Chinese Journal of Urology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the expression of G250 mRNA in patients with renal cell carcinoma (RCC) and its clinical significance. Methods A semi-quantitative reverse transcription (RT)-PCR was developed to assay the levels of G250 mRNA obtained from 7 normal renal tissues and 33 cases of RCC.Of the 33 RCC cases,28 had clear cell carcinoma and 5 had non-clear cell carcinoma.By UICC staging, 8 cases were diagnosed with T_1 stage carcinoma,13 with T_2 and 12 with T_3.Pathological grading showed 11 cases of G_1,15 of G_2,and 7 of G_3. Results Among the 33 cases of RCC,G250 mRNA expression was present in 28 cases (84.8%).However, it was absent in all the 7 normal renal tissues.There was a significant difference between the 2 groups (P0.05);while they were 0.48?0.32,0.23?0.16 and 0.11?0.11,respectively,in RCC cases of G_1,G_2 and G_3,which showed that the expression of G250 mRNA was inversely correlated with tumor grades(P
