Experimental study of the expression of detrusor myosin heavy chain isoforms following bladder outlet obstruction
- VernacularTitle:膀胱出口梗阻引起逼尿肌肌球蛋白重链亚型表达改变的实验研究
- Author:
Hang WANG
;
Guomin WANG
- Publication Type:Journal Article
- Keywords:
Bladder;
Outlet obstruction;
Myosin;
Heavy chain
- From:
Chinese Journal of Urology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the change in expression of detrusor myosin heavy chain isoforms,SM1 and SM2,following bladder outlet obstruction (BOO) in rabbits. Methods Fourteen New Zealand white male rabbits were divided into 2 groups:control group (n=7) and obstruction group (n=7).The bladder necks were partially ligated to form BOO animal model in obstruction group.Then 5 weeks after operation the bladders were resected in both groups, and the weight and volume of the bladders were measured. After the detrusor tissue protein was extracted,SM1,SM2 and their mRNA were tested by western blotting and RT-PCR methods. Results The bladder weights of obstruction and control groups were (13.8?4.4) g and (3.7?0.5) g, respectively (P
