The effects of ascites in severe acute pancreatitis on the normal kidney cells
- VernacularTitle:重症急性胰腺炎腹水对正常肾细胞影响
- Author:
Baoquan CHENG
;
Ning ZHONG
;
Hongbo REN
;
Yanqing LI
- Publication Type:Journal Article
- Keywords:
Pancreatitis?Tumor necrosis factor?Kidney?Apoptosis
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of renal injury in severe acute pancreatitis(SAP).Methods:54 SD rats were randomly divided into sham operation group(n=24) and SAP group(n=30).SAP model was induced by injection of 5% sodium taurocholate solution into bilo-pancreatic duct.Rats from each group were killed and the serum and ascite were collected at 6h,12h,24h after operation.Tumor necrosis factor-?(TNF-?)level of serum and ascite were measured by enzyme linked immuno-sorbent assay (ELISA).The expressions of TNF-? mRNA were assessed by RT-PCR analysis.The serum and ascites that had been collected at 24h after development were injected into medium of NRK cell,then the levels of TNF-? secreted by NRK cell were measured after injection 6h,12h,24h,and the cell apoptosis were also evaluated after injection 24h.NRK cell apoptosis were evaluated by flow cytometry (FCM).Results:①The serum and ascites levels of TNF-? were signficantly elevated in SAP groups compared with sham operation groups at any time point(P
