Gene transfer with Levovist and plasmid GFP into skeletal muscle cell
- VernacularTitle:超声造影剂Levovist介导质粒GFP转染小鼠骨骼肌细胞的实验研究
- Author:
Xinghua WANG
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Contrast media;
Gene transfer;
Muscle,skeletal
- From:Chinese Journal of Ultrasonography
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Levovist to gene transfer in skeletal muscle cells in mice.Methods H2K myoblast cells were treated by ultrasound (1 MHz,1 W/cm~2) with pulse wave (20% duty cycle) using plasmid green fluorescent protein (GFP) in vitro.Exposure duration was 10 s,20 s,30 s,40 s,50 s and 60 s.The trend of transfection efficiency by flow cytometer and cell viability by Trypan blue assays were measured.In vivo studies,transfection efficiencies were assessed by counting GFP positive fibres on mice skeletal muscle.Tissue damage was assessed on serial sections.Results Ultrasound alone showed better trend of transfection efficiency than Levovist in vitro and addition of microbubbles had the effect to reduce cell viability significantly.In vivo,Levovist with or without ultrasound had no effect on gene transfer,instead it increased tissue damage.Conclusions(Levovist showed) no effect in plasmid DNA transfection in skeletal muscle with increased tissue damage in this experimental condition.
