The thymic recent output function in patients with acute promyelocytic leukemia
- VernacularTitle:急性早幼粒细胞白血病患者胸腺近期输出功能情况
- Author:
Shaohua CHEN
;
Yangqiu LI
;
Lijian YANG
;
Suxia GENG
- Publication Type:Journal Article
- Keywords:
TRECs;
Thymic recent output function;
Leukemia;
Real- time PCR
- From:
Cancer Research and Clinic
1997;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the T- cell receptor excision DNA circles (TRECs) level and evaluate the thymic recent output nave T cells function in patients with acute promyelocytic leukemia (APL). Methods Quantitative detection of TRECs in DNA of peripheral blood mononuclear cells (PBMC) from 9 cases with APL were preformed by real- time PCR (TaqMan) analysis. The TRECs- number was related to the number of T- cells by determination of the number of CD-3 positive cells. 14 normal individuals served as controls. Results In comparison with normal individuals [ (4.10?3.65) copies/1000 PBMC and (6.36?5.28) copies/1000 CD+3 cells], a dramatic reduction of TRECs values in patients with APL [ (0.13?0.22) copies/1000 PBMC and (0.77?1.60) copies/1000 CD+3 cells] could be found (P =0.0004, P =0.0005). Conclusions This is, to our knowledge, the first description of TRECs level which was markedly reduced in APL patients.
