Effect of Antioxidant Vitamin Supplementation on Hyperbaric Oxygen Injury in Rats.
- Author:
Chan Kwon JUNG
1
;
Bo Im YOO
;
Kyo Young LEE
;
Chang Suk KANG
Author Information
1. Aeromedical Center, Republic of Korea Air Force, Korea.
- Publication Type:Original Article
- Keywords:
Hyperbaric oxygenation;
Nitric oxide;
Intercelluar adhesion molecule-1;
Antioxidants
- MeSH:
Animals;
Antioxidants;
Ascorbic Acid;
Free Radicals;
Humans;
Hyperbaric Oxygenation;
Intercellular Adhesion Molecule-1;
Lung;
Male;
Nitric Oxide;
Oxygen*;
Rats*;
Rats, Sprague-Dawley;
Vitamin E;
Vitamins*
- From:Korean Journal of Aerospace and Environmental Medicine
2004;14(4):135-141
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Antioxidants vitamin C and vitamin E may protect against the toxic effect of oxygen free radicals that are preferentially produced after exposures to hyperbaric oxygen (HBO). This study investigated the effect of vitamin C and vitamin E on serum nitric oxide (NO) concentration and intercellular adhesion molecule-1 (ICAM-1) expression on lung after HBO exposure. METHODS: Male Sprague-Dawley rats weighing 200 to 250 g were exposed to HBO at 3 ATA of 100% O2 for 3 hours. The experimental groups were given vitamin C (125 mg/day per rat) and/or vitamin E (50 mg/day per rat) orally, from 5 days prior to the HBO exposure to the day of sacrifice. Serum NO concentrations were determined by measuring NO end product nitrite by non-enzymatic Griess assay. Expression of ICAM-1 on lung was observed by immunohistochemical analysis. RESULTS: The serum nitrite levels were significantly increased after HBO exposure and were higher at 24 hours after HBO exposure than at 0 h (P<0.05). The expression of ICAM-1 was weak immediately after HBO exposure and enhanced at 24 hours. There were no pronounced suppressive effects of vitamins on serum NO production and ICAM-1 expression induced by the 3 hours HBO exposure. CONCLUSION: The 3 hours HBO exposure induces the serum NO production and ICAM-1 expression on lung. The short-term supplementation of vitamin C or/and E do not suppress the NO production and ICAM-1 expression on lung.
