Expression of perforin and granzyme B in peripheral blood and tissue specimen during acute rejection of human renal transplantation
- VernacularTitle:穿孔素和颗粒酶B的表达与肾移植术后急性排斥反应的关系
- Author:
Xiaoming DING
;
Xiaodong LIU
;
Puxun TIAN
- Publication Type:Journal Article
- Keywords:
Renal transplantation;
Perforin;
Granzyme B;
Graft rejection
- From:
Chinese Journal of Organ Transplantation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of acute rejection of human renal transplantation and detect the sensitive markers for early diagnosis of acute rejection. Methods Immunohistochemistry was used to detect the expression of Perforin and Granzyme B molecules during acute rejection in the renal grafts. The immunocytochemical ABC assay and flow cytometry ways were used to detect the expression of Perforin and Granzyme B molecules in peripheral blood. Results Perforin and Granzyme B molecules were increased in all tissue specimens and lymphocytes of peripheral blood during acute rejection. There was a correlation between the Granzyme B and histological changes ( P
