Effects of leukemia inhibitor factors on the proliferation and differentiation of cultured human neural stem cells(hNSC) derived from the fetal brains
- VernacularTitle:LIF对人胎脑神经干细胞体外增殖和分化的影响
- Author:
Guocai YIN
;
Zuo LUAN
;
Fengqing YAN
;
Suqing QU
;
Wanli GUO
- Publication Type:Journal Article
- Keywords:
Neural stem cells from human fetal brains;
Leukemia inhibitor factor(LIF);
Proliferation;
Differentiation
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
0.05) . No significant differences were observed between the expansion folds of hNSC derived from various fetal brain samples when cultured under the same conditions. LIF played great roles on cell proliferation,in LIF + groups, hNSC cell number increased ranging from 4 000-8 400 folds, no cell differentiation occurred; and in LIF" groups,only 43 to 96 folds.The differentiation phenomenons were watched when cultured more than two months. In the course of cell culturing, observed that the effects of LIF on hNSC expansion were obviously demonstrated 50-60 days after inoculation.The number of neurons and astrocytes differentiated from the cultured hNSC were respectively identified by means of Immuno-cytochemical fluorescent assay, and the percentages of neurons(as a proportion of neuron and astrocyte number) were calculated,which were ranging from 12% to 83% in LIF+ cultures, significantly higher than 8% to 23% in LIF- ones(P
