Identification of the type and the subtype of interferon with the application of protein biochips
- VernacularTitle:运用蛋白质芯片技术鉴定干扰素的亚型
- Author:
Ping ZHANG
;
Xiaoyan LIU
;
Zhenshan XU
;
Lihua SONG
- Publication Type:Journal Article
- Keywords:
Interferon;
Subtype;
Protein biochip
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a protein biochip flat to identify the subtype of interfon by adopting the 6 mAb which we have prepared.Methods: Recombinant human interferon was onto the NHS modified gold biochips.then the protein biochips were incubated with the 6 mAb.Cy3 conjugated sheep anti-mouse IgG was used to detect the antigen-antibody.Arrays are imaged using a fluorescence scanner (GenePix4100A) at 532 nm for Cy3.This method was compared with the traditional ones:ELISA,Western blot.Results:The results showed that this flat had speciality to identify the subtype of interfon. Conclusion: The protein biochip plat can provide a practical method to identify the subtype of interfon.It also has some advantages:high quantities;simple operation;low sample dosage and high repetition.
