Research on PHA-CIK cells' immunophenotype and cytotoxicity
- VernacularTitle:PHA-CIK细胞的免疫表型和细胞毒活性
- Author:
Fuli QIN
;
Shaolin ZHANG
;
Qiutang ZHANG
;
Yuren XI
- Publication Type:Journal Article
- Keywords:
Cytokine induced killers;
Phytohemagglutinin;
Immunophenotype;
Cytotoxicity
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research PHA-CIK cells' phenotype and cytotoxicity. Methods: Using phytohemagglutinin(PHA)to stimulate peripheral blood mononuclear cells(PBMNCs) for 24 h,then cultured like incubating cytokine induced killers by traditional method. On 15th day examined its immunophenotype by flow cytometry and using MTT method to evaluate cytotoxicity. Results: The ratio of CD3+,CD8+, CD3+ CD8+ and CD3+ CD56+ cells were high.PHA-CK cells cytotoxicity was strong in some degree.Conclusion:PHA-CIK cells had strong cytotoxicity.The result provides an experimental basis for biotherapy.
