The Impact of Apolipoprotein A-I Polymorphisms on the Lipid Profiles in Middle Aged Healthy Men and Women.
10.4070/kcj.2004.34.12.1158
- Author:
Jae Youn MOON
1
;
Eun Young CHO
;
Won Ho KIM
;
Seong Hun CHOI
;
Young Guk KO
;
Hyun Young PARK
;
Jong Ho LEE
;
Jong Eun LEE
;
Yang Soo JANG
Author Information
1. Division of Cardiology, Cardiovascular Center, Yonsei University College of Medicine, Seoul, Korea. Jangys1212@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Apolipoprotein A-I;
Polymorphism;
Triglyceride;
HDL-cholesterol
- MeSH:
Alleles;
Apolipoprotein A-I*;
Apolipoproteins*;
Female;
Genotype;
Humans;
Linkage Disequilibrium;
Lipoproteins;
Male;
Middle Aged*;
Plasma;
Triglycerides
- From:Korean Circulation Journal
2004;34(12):1158-1166
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Apolipoprotein A-I is the major lipoprotein constituent of high density lipoprotein in plasma. In this study, the role of two polymorphisms in the apo A-I gene was investigated on the serum lipid profiles and apo A-I levels in healthy men and women. SUBJECTS AND METHODS: Blood samples were obtained from 417 subjects (M : F=169 : 248, mean age 47.2 years). The apo A-I genotypes were determined by SNP-IT assays using the SNPstream 25KTM system. RESULTS: The frequencies of the A allele at the XmnI restriction site and position -75 bp were 0.25/0.23 and 0.19/0.17 in men and women, respectively. A strong positive linkage disequilibrium (D'=0.990) between two polymorphisms was detected. In men, the A allele at the XmnI restriction site was associated with significantly lower levels of triglyceride (p=0.028) compared to the G/G subjects, but no significant associations were detected between the G-75A polymorphism and any of the lipid traits examined. In women, each A allele for the XmnI restriction site and -75 bp polymorphisms were significantly associated with higher levels of apo A-I (p=0.032 and p=0.012). In the multiple regression analysis, the HDL, being a current drinker and the A allele of the XmnI restriction site polymorphism were major determinants of the serum apo A-I levels in women (R2=0.272, p<0.05). CONCLUSION: Our study showed that the A allele at XmnI restriction site in the apo A-I gene was associated with decreased triglyceride levels in men. Each A allele of two polymorphisms was associated with an elevated apo A-I level in women.
