Analysis of multiple alternative spliceosome of nfic gene in postnatal rat molar tissues
- VernacularTitle:出生后大鼠牙胚组织中nfic基因多种选择性剪接体的克隆研究
- Author:
Xianghui XING
;
Lingying WEN
;
Yan JIN
- Publication Type:Journal Article
- Keywords:
Nuclear factor I-C;
Tooth development;
Alternative spliceo some
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the multiple alternative sp l iceosome of nfic gene in postnatal rat molar tissues. Methods: 3 d postnatal SD rat molar tissues were removed and mRNA was obtained by magne tic beads coupled with oligo-dT18. Two pairs of primers specific to the nfic gene were designed, and nfic gene in the rat molar tissue was amplified b y RT-PCR method.The fragments were inserted into competent DH5? bacteria.Posit ive clones were selected randomly and evaluated by enzyme digestion and sequenci ng.Results:Three alternative spliceosomes of nfic gene with the size of 1.5 kb,900 bp and 650 bp respectively were obtained. The spliceosome s were named rNFIC-1、 rNFIC-2 and rNFIC-3 respectively.Conclusion s:The nfic gene is expressed in postnatal rat molar, and there is a multiple alternative splicing way for the nfic gene to play function.
