Cloning of nm23-H1 cDNA gene and construction of recombinant adenovirus vector
- VernacularTitle:nm23-H1cDNA克隆及腺病毒载体的构建
- Author:
Gang SUN
;
Zhan YANG
;
Longjiang LI
- Publication Type:Journal Article
- Keywords:
Gene;
nm23-H1;
Adenovirus;
Vector;
Squencing
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone human nm23-H1 c DNA gene and to recombine it into adenovirus vector.Methods:Nm23 -H1cDNA fragment was amplified from human liver by reverse transcription-polym erase chain reaction(RT-PCR) and cloned into pMD18-T plasmid. The gene was exa mined by sequencing. Then pAd-Shuttle-CMV vector containing nm23-H1 was const ructed. Results:The results showed that the gene fragment cloned in pMD18-T was coincident with the sequence of nm23-H1.A pAd-Shuttle -CMV v ector containing nm23-H1 gene with correct order was con firmed by Kpnl digestion. Conclusion:nm23-H1 may be cloned into recombined adenovirus vector pAd-Shuttle-CMV.
