Study of natural killer cell stimulatory receptor NKG2D and its ligand MICA in patients with epithelial ovarian tumors
- VernacularTitle:卵巢肿瘤患者自然杀伤细胞活化性受体NKG2D与其配体MICA表达的初步研究
- Author:
Min LIU
;
Beihua KONG
;
Xun QU
- Publication Type:Journal Article
- Keywords:
Ovarian cancer;
Peripheral NK cells;
NKG2D;
MICA
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of NKG2D in peripheral blood of patients with ovarian benign or malignant tumors, and the expression of the human MHC class Ⅰ chain-related gene A(MICA) on the correspondent tumor tissues. To analyze and discuss the function of NKG2D in anti-ovarian cancer mechanism and its relation with immune escaping of cancer.Methods:Flow cytometry analysis was used to detect NKG2D in the peripheral blood of 42 ovarian carcinoma patients, 23 ovarian tumor patients and 20 health persons. The expressions of MICA in part of the correspondent tissues were examined by means of reverse transcription-polymerase chain reaction (RT-PCR).Results:The expression of NKG2D were (94 23?6 02)%?(98 70?0 98)%?(98 61?1 59)% respectively, compared with the other two groups, the NKG2D expression in malignant group was significantly low; The rate of MICA mRNA expression in ovarian carcinomas was significantly higher than that in benign and normal tissues. No evident relation was found between the expression of MICA mRNA and the clinical factors.Conclusion:The activity of NK cell and the anti-cancer cellular immunity level reduce in patients with ovarian cancer; The decrease of the receptor NKG2D is a reason for the descend of the activity of NK cells; MICA mRNA expression is relative to malignant transformation; the immune-escape of ovarian cancer probably is relative to the down-regulation of NKG2D and the up-regulation of its ligand MICA.
