Study on Estrogenic Effect of Organic Extracts of Reclaimed Water in Tianjin
- VernacularTitle:再生水有机提取物雌激素效应研究
- Author:
Keming LIU
;
Lei ZHOU
;
Chunhua WANG
- Publication Type:Journal Article
- Keywords:
Water pollution;
Cell proliferation assay;
Recombined yeast assay;
Reclaimed water;
Estrogenic effect
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the estrogenic effect of organic extracts of effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Methods Reverse phase C-18 solid-phase extraction (RP-C18SPE) was used for the extraction of target compounds from water samples. Then, a simplified proliferation test with human estrogen receptor-positive MCF-7 breast cancer cell and recombined yeast assay were applied to assess the estrogenic effect in effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Results The amount of extracts of reclaimed water inducing the maximum effect in cell proliferation assay was equal to that from 1.00 ml primary water. In recombined yeast assay, the amount of extracts of reclaimed water inducing maximum ?-galactosidase activity was equal to that from 20.00 ml primary water. Conclusion The effluent of reclaimed water treatment plant still shows estrogenic effect though slightly reduced.
