Expression of Translation Elongation Factor 1?1 in 16 HBE Cells Induced by Anti-Benzo(a)pyrene-7, 8-diol-9, 10-epoxide
- VernacularTitle:反式二羟环氧苯并芘诱导人支气管上皮细胞翻译延长因子1?1基因的表达
- Author:
Shejuan AN
;
Jiakun CHEN
;
Xuemin CHEN
- Publication Type:Journal Article
- Keywords:
Benzo(a)pyrene;
Translation elongation factor 1?1;
Suppression subtractive hybridization;
Bioinformatics
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of translation elongation factor 1?1 in anti-BPDE transformed and carcinoma 16 HBEs. Methods Suppression subtractive hybridization (SSH), bioinformatics and semi-quantitative RT-PCR were applied. The cDNA of anti-BPDE transformed and carcinoma 16 HBE cells were used as tester respectively, and the cDNA of normal 16 HBE was used as driver, the library of subtractive hybridization were profiled and inverted into TA cloning vector after two times of hybridization and two times of PCR. After the screening, sequencing and analysis of sequences, semi-quantitative RT-PCR was performed accompanying to the inner reference of ?-actin. Results 9 differentially expressed fragments were consistent with translation elongation factor 1?1 in different regions in Genbank, and the expressions were up regulated in BPDE transformed and carcinoma 16 HBE cells. Conclusion Translation elongation factor 1?1 may be related to the transforming effect and carcinogenesis of anti-BPDE.
