Effects of MPA, sirolimus, FTY720 on human pancreatic islet function in vitro
- VernacularTitle:霉酚酸、雷帕霉素及FTY720在体外对成人胰岛功能的影响
- Author:
Xiangfeng CHEN
;
Zhihong LIU
;
Weiping DONG
- Publication Type:Journal Article
- Keywords:
Islets of langerhans;
Immunosuppressive agents;
Toxicity tests
- From:
Chinese Journal of Organ Transplantation
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of three immunosuppressive agents on the function of human adult islets of langerhans in vitro, and explore the suitable immunosuppressive protocols for human islet transplantation.Methods In vitro, the isolated and purified islets were incubated with the immunosuppressive agents (mycophenolic acid, sirolimus, and FTY720) at different concentrations for 24 h, then stimulated by glucose at low ((2.8) mmol/L) and high ((16.7) mmol/L) levels. The (insulin) release after stimulations was detected using ELISA kit, and stimulation index was calculated.(Results) In control group (no agents exposure), the insulin release were ((7.37)?(1.74)) ng/ml at low (level) and ((15.15)?(5.39)) ng/ml at high level respectively, with stimulation index achieved to (2.06)?(0.46). Compared to control group, treatment with mycophenolic acid led to dramatic reduction of (insulin) release (P
