Genitofemoral nerve graft repair the ablated cavernous nerves in rats
- VernacularTitle:生殖股神经移植重建大鼠勃起神经通道
- Author:
Xinjun SU
;
Liquan HU
;
Xinmin ZHENG
;
Shiwen LI
;
Binqun TIAN
- Publication Type:Journal Article
- Keywords:
Erectile dysfunction;
Nerve fiber;
Insulin like growth factor;
Rat
- From:
Chinese Journal of Urology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the feasibility of repairing surgically ablated cavernous nerves by using an interposition genitofemoral nerve graft and with nerve growth enhancing media (insulin-like growth factor-I,IGF-I) injection.MethodsA total of 54 male adult Sprague-Dawley rats were randomly divided into 3 groups (each of 18 rats),including sham operation controls, bilateral cavernous nerve ablation and nerve graft with IGF-I injection groups.At 1,3 and 6 months after surgery, the rat models were evaluated with Apomorphine Test.Then nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining was used to identify nNOS in penile nerve fibers of the proximal portion of penile shaft.ResultsAt 1,3 and 6 months after surgery, the rats of sham operation group had normal erectile function (erection rate,100%),while the rats of nerve ablation group lost erectile function at all (erection rate,0%).There was no statistically significant difference of ED between the nerve ablation and nerve graft groups at 1 month;however,at 3 and 6 months,Apomorphine Test resulted in tumescence of 50% and 66.7% of the nerve graft groups vs 0% and 0% of the nerve ablation groups, respectively ( P
