Research on IGF-Ⅱprotein expression in lung cancer by tissue microarray
- VernacularTitle:应用组织微阵列技术研究肺癌组织中IGF-Ⅱ的表达
- Author:
Congzhong ZHU
;
Xinyun WANG
;
Ting LIU
- Publication Type:Journal Article
- Keywords:
lung neoplasm;
lung cancer;
tissue microarray;
tissue chip;
IGF-Ⅱ;
immunohistochemical
- From:
China Oncology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:Constructing a high-flux tissue microarray/tissue chip and detecting IGF-Ⅱprotein expression of cases by it, and to determine the correlation between IGF-Ⅱprotein expression and lung cancer. Methods:A series of tissue chips were prepared by using tissue arrayer with samples from lung cancers of different histological classifications. Specimens from 54 cases of lung cancer and 10 cases of normal lung tissues were detected immunohistochemically on a tissue chip for IGF-Ⅱprotein expression and its correlation to clinic-pathological parameters was analyzed statistically. Results:Positive rate of IGF-Ⅱprotein in lung cancer was 42.6%(23/54), which was higher than that of normal lung(0.0%, 0/10, P0.05). Conclusions:IGF-Ⅱprotein might be related to the malignant behaviors of lung cancer. Detecting the expression of IGF-Ⅱ protein probably can predict the prognosis of lung cancer. It is feasible to utilize tissue chip for screening of clinical tissue specimens on a large scale.
