Lipotoxicity of palmitic acid on islets and protecting effect of fenofibrate
- VernacularTitle:棕榈酸对胰岛的脂毒性及非诺贝特的保护作用
- Author:
Li ZHANG
;
Ling GAO
;
Jun LIANG
;
Jiajun ZHAO
- Publication Type:Journal Article
- Keywords:
Islets of Langerhans;
Palmitic acid;
Procetofen (fenofibrate);
Insulin;
Pancreas-duodenum homeobox-1;
Glucose transport proteins
- From:
Chinese Journal of Endocrinology and Metabolism
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of palmitic acid (PA) and fenofibrate (FF) on rat islets. Methods Rat islets were isolated with collagenase digestion and divided into 6 groups: control group, PA0.2 group (with 0.2 mmol/L PA), PA0.4 group(with0.4mmol/LPA),FF group (with 5?10 -6 mol/L fenofibrate), PA0.2+FF group and PA0.4 +FF group. After being cultured 24 hours with PA in the presence or absence FF, baseline insulin secretion (BIS) and glucose stimulated insulin secretion (GSIS) were examined. The mRNA levels of insulin(INS), pancreas-duodenum homeobox-1 (PDX-1), glucose transport protein 2 (GLUT-2) and PPAR? were determined by RT-PCR or real-time PCR. Results (1)In both PA0.2 and PA0.4 groups, BIS was increased and GSIS increase was impaired as compared with control group (P0.05). Expressions of INS, PDX-1 ,GLUT2 and PPAR? mRNA were enhanced in PA0.2 +FF group and PA0.4 +FF group as compared with the two groups without FF respectively (all P
