Construction of Fab fragment phage antibody library and selection of clones expressing anti-human ?_1-AR antibody
- VernacularTitle:人源性Fab段噬菌体抗体库的构建及抗人?_1-AR抗体克隆的筛选
- Author:
Runqiu ZUO
;
Shujin ZHAO
;
Jie WANG
;
Hongbin ZHANG
;
Jian QIU
;
Dingcheng XIANG
- Publication Type:Journal Article
- Keywords:
?_1-adrenoceptor;
Dilated cardiomyopathy;
Phage antibody library;
Phage display technology;
Fab fragment antibody
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a human Fab phage antibody library and to obtain some recombinant clones which can express Fab fragment antibody against ? 1-adrenergic receptor.Methods:Fd heavy chain gene and ??? light chains gene of IgG obtained by RT-PCR from peripheral lymphocytes of DCM patients whose anti ? 1-AR antibodies are present were cloned into pComb3 vector and the human Fab phage antibody library was constructed.The library was panned by phage display technology with ? 1-ARECⅡ as antigen.Results:A human Fab phage antibody library with 1.4?10 6 capability was constructed successfully,a positive clone against human ? 1-AR was screened from the phage antibody library.Conclusion:The recombinant clones which express Fab antibody against ? 1-AR can be obtained by phage display technology.
