Altered HA308-317 peptides inhibit HLA-DR4 specific T cell response
- VernacularTitle:HA308-317变构肽对HLA-DR4特异性T细胞活化的抑制作用
- Author:
Xia LI
;
Qiang ZHOU
;
Ru LI
;
Zhanguo LI
- Publication Type:Journal Article
- Keywords:
HLA-DR4;
Altered peptides ligand;
Influenza virus haemagglutinin;
Type Ⅱ collagen;
T cell activation
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the inhibitory effect of altered HA308-317 peptides on HLA-DR4 restricted CII specific T cell response.Methods:Three altered HA308-317 peptides and CII263-272 were synthesized using solid-phase techniques. The binding of altered HA308-317 peptides for HLA-DR4 molecules was assayed using flow cytometry. The suppressive effect of altered HA308-317 peptides on CII-mediated T cell proliferation was determined using 3H incorporation assay. The level of IL-2 in the supernatants was identified by ELISA. The expression of CD25 and CD69 on T cell surface were studied using flow cytometry.Results:The altered HA308-317 peptides were able to bind to HLA-DR4 molecules and competed with CII263-272. Altered HA308-317 peptides inhibited T cell proliferation and IL-2 production induced by CII263-272( P
