Expression of TLR4 and TLR2 mRNA in the peripheral blood mononuclear cells of the patients with Gram negative or Gram positive infections
- VernacularTitle:革兰氏阴性、阳性菌感染后外周血单个核细胞TLR4、TLR2的表达
- Author:
Xiaoyan SHEN
;
Feng XUE
;
Xiaohong CHEN
;
Jie ZHENG
- Publication Type:Journal Article
- Keywords:
Toll-like receptor;
Gram negative infection;
Gram positive infection;
Quantitative real-time PCR
- From:
Chinese Journal of Immunology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of TLR4 mRNA?TLR2 mRNA in the peripheral blood mononuclear cells (PBMC) from the patients with Gram positive and Gram negative infections.Methods:TLR4 and TLR2 mRNA were detected in the patients with Gram negative infections ( n =20) and the patients with Gram positive infections ( n =15) and the normal persons as control ( n =24) with the method of Taqman Real-time PCR.Results:TLR4 mRNA expression in the patients with Gram negative infections were significantly higher than Gram positive infections patients and the normals ( P 0.05).Conclusion:The results showed that the increased expression levels of TLR4 mRNA in PBMC from the Gram negative infectious patients are associated with Gram negative infections and the detection of TLR4 mRNA expression may be a method of diagnosis for Gram negative infections in the early stage.
