Prevention of cyclosporin A induced chronic nephrotoxicity in rats with cationic liposome-mediated antisense TGF-?1 oligodeoxynucleotides
- VernacularTitle:转化生长因子-?1反义寡核苷酸减轻环孢素A对大鼠的慢性肾毒性
- Author:
Qian ZHAO
;
Yinpu ZHANG
;
Chibing HUANG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-?;
Oligonucleotides, antisense;
Cyclosporine
- From:
Chinese Journal of Organ Transplantation
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of inhibition of transforming growth factor-beta 1 (TGF-?1) expression by liposome-mediated antisense oligonucleotides on chronic nephrotoxicity induced by cyclos-porin A in rats. Methods Rats were on low salt diet and CsA was administered once every day by intraper-itoneal injection (15 mg/kg every day) for 4 weeks. Antisense oligodeoxynucleotides for TGF-?1 were introduced into the nephritic kidney by liposome-mediated gene transfer method to inhibit the overproduction of TGF-?1. Results Under the condition of low salt diet, a rat model of CsA-induced nephrotoxicity was established. The changes of level of serum creatinine and characteristic histopathology of proximal tubular injury and tubulointerstitial fibrosis were observed. In the liposome-mediated AODN rats, the expression of TGF-?1 mRNA was decreased by RT-PCR (P
