Donor ICAM-1 expression in cardiac xenografts
- VernacularTitle:大鼠接受小鼠异种心脏移植后粘附分子ICAM-1的表达
- Author:
Yi LIU
;
Zongquan SUN
- Publication Type:Journal Article
- Keywords:
Heart tranplantation;
Intercellular adhesion molecule-1;
Graft rejection
- From:
Chinese Journal of Organ Transplantation
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the pathological characteristics of delayed xenograft rejection and the expression of donor ICAM-1 in mouse-to-rat cardiac xenografts. Methods BALB/c mice and Lewis rats served as donors and recipients respectively. The model of mouse-to-rat heterotopic heart xenotransplantation was established. The cardiac xenografts were harvested at 12 h, 24 h, 36 h, 48 h after transplantation and at the time when no pulsations could be detected in the transplanted heart respectively. The normal BALB/c mouse hearts were harvested as control group. The grafts were col lected to receive pathological and immunohistochemical examinations as well as to detect the level of ICAM-1 mRNA in the xenografts. Quantitive measurement of ICAM-1 expression in the grafts was done by using multimedia pathology imaging analysis system. RT-PCR products of xenografts were separated by agrose gels and the densities of the bands were determined by density scanning. Results The pathologic examination of xenografts showed hyperemia, hemorrhage with inflammatory cells infiltrated at 12 h after transplantation and they became more and more serious as time went on. The pathologic examination of rejected xenografts showed widespread intravascular thrombosis, hyperemi-a, hemorrhage, coagulative necrosis with a large number of inflammatory cells infiltrated. The stained color of vascular endothelial cells and cardiac myocytes was significantly more intensive in the xeno-grafts than that of normal BALB/c mouse hearts in the control group. The relative density values (ICAM-1/?-actin) were also significantly higher in the xenografts than that of the control group. Conclusion ICAM-1 expression in the xenografts was up-regulated, which was related with the development of the delayed xenograft rejection.
