The selective killing effect of adenoviral mediated HSV-tk/GCV suicide gene system controlled by hTERT promoter on bladder cancer cells
- VernacularTitle:人端粒酶逆转录酶启动子调控腺病毒介导的胸苷激酶基因治疗膀胱癌的实验研究
- Author:
Yaxuan WANG
;
Wenqing CAI
;
Wei LI
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Carcinoma;
Adenoviruses;
hTERT;
HSV-tk gene
- From:
Chinese Journal of Urology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the selective killing effect of adenoviral mediated herpes simplex virus-thymidine kinase/ganciclovir (HSV-tk/GCV) suicide gene system controlled by human telomerase reverse transcriptase (hTERT) promoter on bladder cancer cells in vitro. Methods Bladder cancer cell line 253J and human fibroblast cell line MRC-5 were transfected by the recombinant adenovirus of different multiplicities of infection (MOI),and the infection rate was measured by observing the expression of enhanced green fluorescent protein (EGFP) under the fluorescent microscopy.Ad-hTERT-HSV/tk and Ad-CMV-HSV/tk were transduced into 253J and MRC-5,followed by GCV treatment. The relative survival rate of cells in presence of prodrug GCV was measured with MTT method. Results Recombinant adenovirus Ad-hTERT-EGFP could selectively infect 253J cells,with the infection rate associated with the increasing MOI of recombinant adenovirus (P
