Effects of angiotensin Ⅱ on apoptosis and apoptosis genes in vascular endothelial cells and the influences of enoxaparin on the effects
- VernacularTitle:低分子肝素对血管紧张素Ⅱ所致体外血管内皮细胞凋亡及调控基因变化的干预
- Author:
Xuguo LIANG
;
Hongying CHEN
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅱ;
Heparin, Low-molecular-weight;
Apoptosis
- From:
Chinese Journal of Geriatrics
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of angiotensin II in its different concentrations and different action time on VEC apoptosis and the expression of Fas, Bcl-2 in vascular endothelial cells (VEC) and to study the influences of enoxaparin on the above roles. Methods Flow cytometer was used to measure the apoptosis ratio and the expression of Fas, Bcl-2 which were induced by angiotensin II in its different concentrations and different action time, and influenced by enoxaparin. Results Apoptosis ratio(1.86?0.18,30.62?4.20;1.67?0.15,30.08?4.41)and the expression of Fas(1.13?0.16,14.85?1.47;1.13?0.16,15.67?1.97), Bcl-2 (2.38?0.43,9.62?1.81;2.38?0.43,12.33?1.73)were induced and increased by angiotensin II with the increase of concentrations and action time. Enoxaparin had an effect on apoptosis ratio and the expression of Fas, Bcl-2, which were induced by angiotensin II(30.62?4.20,18.31?2.64;14.85?1.47,7.28?1.36;9.62?1.81,16.05?1.79). Conclusions Apoptosis ratio and the expression of Fas, Bcl-2 may be induced and increased by angiotensin II with the increase of the concentrations and the action time. Enoxaparin may have the reduce effect on apoptosis ratio and the expression of Fas and Bcl-2 in vascular endothelial cells.
