Immunogenicity of Porphyromonas gingivalis gene vaccine pcNDA 3.1(+)/kgp_(cd)
- VernacularTitle:牙龈卟啉单胞菌基因疫苗pcDNA3.1(+)/kgp_(cd)免疫原性研究
- Author:
Fengqiu ZHANG
;
Lianjia YANG
;
Zhifen WU
- Publication Type:Journal Article
- Keywords:
Porphyromonas gingivalis;
Gene vaccine;
Submandibular gland-targeted injection;
Animal immunization
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the immunogenicity of the recombinant plasmid pcDNA 3.1(+)/kgp_ cd.Methods:BALB/c mice were immunized with recombinant plasmid pcDNA 3.1(+)/kgp_ cd,KGP_ cd protein(control) or vector pcDNA 3.1(+)(control) by quadriceps injection or targeted submandibular gland(TSG) injection. Serum IgG and salivary sIgA levels were assayed by indirect ELISA after immunization. The expression of the protein KGP_ cd in quadriceps and submandibular gland was detected by immunohistochemistry techniques.Results: Serum specific anti-KGP_ cd IgG elicited by pcDNA 3.1(+)/kgp_ cd and KGP_ cd protein was significantly higher in both the quadriceps injection group and the TSG group than that in the pcDNA 3.1(+) group (P
