Effect of mycophenolic acid on the proliferation and MCP-1 secretion in the glomerular mesangial cells
- VernacularTitle:霉酚酸对小鼠肾小球系膜细胞增殖及分泌单核细胞趋化因子-1的影响
- Author:
Yuehui ZHANG
;
Jingding WANG
;
Feng QIU
- Publication Type:Journal Article
- Keywords:
Mycophenolic acid;
Kidney glomerulus;
Cell division;
Monocyte chemoattractant proteins
- From:
Chinese Journal of Organ Transplantation
1996;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitory effect of mycophenolic acid (MPA) on the proliferation and monocyte chemoattractant protein-1 (MCP-1) secretion in mice glomerular mesangial cells (GMCs).Methods MPA with different concentrations ( 0.1 , 1, 5 and 10 ?mol/L ) was added into the cultured mice GMC line in vitro for 24 h. The GMCs proliferation level was detected by the 3-(4,5-Dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) incorporation. The absorbance for counting the GMCs survival was read at 570 nm by using an automated microplate ELISA reader. The cells treated with MPA (0, 0.5 and 2.5 ?mol/L ) were stimulated by TNF-? (20 ng/ml), IL-1? (2 ng/ml), IFN-? (10 ng/ml). Four h later, the MCP-1 concentration in supernatant was determined by ELISA. At the same time, the lymphocyte migration was examined.Results MPA has a concentration -dependent antiproliferative effect on cultured GMCs. The secretion of MCP-1 of cultured GMCs stimulated by TNF-?, IL-1?, IFN-? was significantly increased. Addition of MPA could signi -ficantly inhibit the secretion of MCP-1 ( P
