Toxic Effect of Exhaust Particles Extracts of Diesel Vehicles on V79 Cell
- VernacularTitle:柴油车尾气颗粒物提取物对V79细胞的毒性
- Author:
Wenbiao LIU
;
Shengzhang DONG
;
Zhongning LIN
- Publication Type:Journal Article
- Keywords:
Diesel exhaust particles extracts;
Lactate dehydrogenase;
Oxidative stress
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the toxicity of diesel exhaust particles extracts(DEPE) on V79 cell in cell viability, membrane and oxidative stress. Methods Cell exposed different concentrations of DEPE for different time , the change of cell viability, the leakage of intracellular lactate dehydrogenase(LDH), intracellular glutathione(GSH) and glutathione peroxidase (GPX)content were determined respectively, and analyzed the relation between GSH and GPX. Results The results showed that the viability of V79 cell decreased and the leakage rate of lactate dehydrogenase increased gradually in according with the increasing dosage of DEPE,and that DEPE could impair V79 cell by oxidative stress, included intracellular GSH content decreased and the activity of GPX increased, indicating that GPX might play a important role in DEPE induced GSH depletion. Conclusion DEPE may impair cell viability and the cell membrane integrity, also impair cell by oxidative stress.
