Determination of Trace Iron(Ⅲ) in Human Hair by Emulsifying Agent OP-XO Spectrophotometry
- VernacularTitle:人发中痕量铁的乳化剂OP-二甲酚橙分光光度测定法
- Author:
Juan DU
- Publication Type:Journal Article
- Keywords:
Spectrophotometry;
Iron;
Emulsifying agent OP
- From:
Journal of Environment and Health
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To make a method to determine trace iron (FeⅢ)in human hair. Methods Based on the coloured reaction between XO and Fe(Ⅲ)in the medium of HAc-NaAc(pH=3.2)with the presence of OP, FeⅢ was determined by spetrophotometry. Results It was found that when pH=3.2 and in the presence of OP, iron(Ⅲ) reacted with XO to form a stationary complex. The maximum absorption wave of Fe(Ⅲ)-XO was 570 nm, as OP was added, it moved to 590 nm, and the sensitivity was increased obviously when the molar absorptivity of the complex reached 3.67?105 L/(mol?cm) from 3.04?104 L/(mol?cm), the linear range for the content of Fe(Ⅲ)was 0.0～0.8 ?g/ml(0.0～20.0 ?g/25 ml), the regression equation of calibration graph was A=0.00236+0.542c(?g/25 ml)with a correlation coefficient of 0.997 6, detection limit was 6.09?10-6 g/L, RSD=0.97% and rate of recovery was 96%-99%. Conclusion The method can be used for the determination of trace iron(Ⅲ)in human hair samples.
