Protective effect of both human TH and GDNF cDNA-engineered SH-SY5Y cells on primary dopaminergic neurons
- VernacularTitle:人酪氨酸羟化酶和神经营养因子双转基因工程细胞对原代多巴胺能神经元的保护作用
- Author:
Wenhua ZHANG
;
Xiaomin WANG
;
Chengyuan WU
- Publication Type:Journal Article
- Keywords:
Parkinson′s disease;
Dopamine;
Tyrosine 3-monooxygenase;
Nerve growth factor;
Genetic engineering
- From:
Chinese Journal of Geriatrics
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct genetically engineered cells that secrete human TH and GDNF at the same time by stable co-transfection pcDNA3.0/hTH and pcDNA3.1/hGDNF in SH-SY5Y cells and study their effects on the gene therapy of Parkinson′s disease (PD). Methods pcDNA3.0/hTH and pcDNA3.1/hGDNF were constructed by inserting human TH and GDNF cDNA into pcDNA3.0 and pcDNA3.1, respectively. SH-SY5Y cells were transfected with pcDNA3.0/hTH and pcDNA3.1/hGDNF and the positive cell clones of human TH and GDNF cDNA engineered cells could be identified by RT-PCR. The engineered cells were co-cultured with primary dopaminergic neurons in mouse. The number and growth condition of primary dopaminergic neurons were examined by Immunocytochemistry. Results The number of primary dopaminergic neurons protected by double-gene engineered cells increased at least by 2.8 times in comparison with the control cells(P
