The effect of focal cerebral ischemic preconditioning on brain edema and the expression of NF-?B and its target gene MMP-9 in rats
- VernacularTitle:局灶性缺血预处理对鼠脑核因子-?B基质金属蛋白酶-9基因表达及脑水肿的影响
- Author:
Yuman HAO
;
Zuming LUO
;
Li GAO
;
Zhong ZHANG
;
Wei CENG
- Publication Type:Journal Article
- Keywords:
Ischemic preconditioning;
NF-kappaB;
Matrix metalloproteinases;
Brain edema
- From:
Chinese Journal of Geriatrics
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of focal cerebral ischemic preconditioning (IPC) on brain edema and the expression of nuclear factor-?B( NF-?B) and its target gene MMP-9. Methods Forty-five SD rats were divided into 3 groups in which control group received sham surgery only, and the other two groups received 2 hours of middle cerebral artery occlusion (MCAO) followed by 22 hours of reperfusion with or without 10 minutes of IPC 3 days before. Brain water content, expression of NF-?B and MMP-9 mRNA were evaluated in each group by wet-dry weight method, immunohistochemistry staining and RT-PCR. Results Compared with the SS group, there was a lower NF-?B immunoreactivity and MMP-9 mRNA level (16 098.2?1 265.3 vs 23 565.8?1 978.4,50.7% vs 84.1%, P
