Comparison of the two methods for screening osteosarcoma cell sublines with different metastatic potential in vitro
- VernacularTitle:两种体外筛选不同转移潜力骨肉瘤细胞亚系方法的比较
- Author:
Xiaobing SHI
;
Anmin CHEN
;
Xianhua CAI
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
metastasis;
cell sublines;
sc reening]
- From:China Oncology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To look for an ideal screening method in vitro for establishing osteosarcoma cell sublines with different metastat ic potential. Methods:20 osteosarcoma cell sublines were isolated preliminari ly by clone technique in vitro. They were screened by electrophores migratio n rate assay and cell migration assay in vitro and obtained respectively a h igh and low metastatic potential osteosarcoma cell sublines (A1、A2、B1 and B2). The advantages of the two methods were compared and confirmed by using cell pro liferation, agarose clony-formation and transplantation in nude mice. Results:The cell proliferation rate , agarose clony-formation ability and spontaneous metastatic ability to lung of A1 and B1 were obviously h igher than that of A2 and B2 and the difference was statistically significant( P
