The experimental study of the biological activity and anti-tumor effects of CD_3AK cells in human umbilical cord blood
- VernacularTitle:人脐血CD_3AK细胞生物活性及抗瘤作用的实验研究
- Author:
Xucang WEI
;
Peini XING
;
Wenli ZHAO
;
Xinhui ZHAI
;
Meisheng LI
;
Liang GUO
- Publication Type:Journal Article
- Keywords:
rhIL-2;
CD 3McAb;
cord blood;
CD 3AK;
anti-tumor;
biological activity
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce human mononuclear cell of cord blood into CD 3 activating killing (CD 3AK) cells with anti-CD 3 monoclonal antibody (CD 3McAb) and recombinant human interleukin-2 (rhIL-2), so that their proliferative activity, activity of killing action, phenotypes and level of secretory cytokines can be observed dynamically. Methods The increase of the number of cells was counted by Tapan-blue staining. The killing action can be measured by using methyl -thiazolyl-tetrazolium-array. The phenotypes of cells were analysed by using indirect immunofluorescence assay. The levels of IL-6, interferon-? (IFN-?) and tumor necrotic factor-? (TNF-?)in culture supernatants were analysed by using enzyme-linked irnmunosorbent assay(ELISA). Results The increase of the number of CD 3AK cells from cord blood was the highest amounting to 78.56 times in the second week. The killing action reached the peak on day 12, and all target cells (malignant cell lines) could be killed significantly. The heterogeneous phenotypes of CD 3AK cells showed that the number of cells with CD 3+, CD 8+, CD 25+, CD 38+, CD 16+ and CD 56+ increased significantly on day 7,14 compared with those of pre-culture (P
