Protein chip technology used in examination for eight autoantibodies and its methodological evaluation
- VernacularTitle:蛋白芯片技术对8种自身抗体的检测及其评价
- Author:
Jinfang SHI
;
Guohao GU
;
Jianqi CHEN
;
Hongxing XU
;
Daren YANG
;
Jitao YU
- Publication Type:Journal Article
- Keywords:
protein chip technology;
autoimmune disease;
autoantibody;
indirect immunoflurescence;
immunoblotting;
gold-colloid assay
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the sensitivity and speciality of protein chip technology, and discuss its value in diagnosis or classification of autoimmune diseases, and to make its methodological evaluation. Methods The anti-dsDNA was detected with gold-colloid assay, indirect immunoflurescence(IIF) assay and protein chip technology, respectively; the other seven autoantibodies including anti-SSA, anti-SSB, anti-Sm, anti-u1RNP, anti-Rib-P, anti-Scl-70 and anti-Jo-1 were simultaneously detected with immunoblotting(IBT) assay and protein chip technology, and then all the results were delt with statistical method. Results For anti-dsDNA, the sensitivity of protein chip technology was better than that of gold-colloid assay; there was significant difference between protein chip technology and IBT assay in detecting anti-Jo-1 in DM/PM(P
