Biological responses of osteoblast-like cells in three-demensional culture under mechanical stretch
- VernacularTitle:牵拉应力对三维培养的成骨样细胞的生物学效应
- Author:
Pengcheng WANG
;
Yingze ZHANG
;
Baicheng CHEN
- Publication Type:Journal Article
- Keywords:
Osteoblasts;
Cell culture;
Stress, mechanical;
Alkaline phosphatase
- From:
Chinese Journal of Orthopaedics
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to investigate the effectiveness of unique cyclic biomechanical stretch on proliferation, alkaline phosphatase (ALP) activity and osteopontin (OPN) mRNA of colonal murine osteoblast-like cell line MC3T3-E1 in three-demensional culture. Methods Gelatin sponges with a size of 2.00 cm?2.00 cm?0.25 cm were used as the three-demensional culture vector of MC3T3-E1, 100 ?l cell suspension was applied to the surface of sponge, the cell number was 1.25?105. The gelatin sponges could be stretched to 5% with 60 cycles/min and 15 min/h on computerized bio-stretch controller. At 2, 4, 6, 8 and 10 d after stretch stimulation, three samples were collected at each time point from the stretch and con-trol group respectively. Cell counting, activity assay of ALP both of cells and medium as well as OPN mRNA measurement were carried out. Results The cell number of the stretch group was more than that of control group from 2 d (P
