Expression and characterization of an EGF-IL-18 fusion protein for targeting (therapy) in insect cells
- VernacularTitle:用于肿瘤靶向治疗的EGF-IL-18融合蛋白在昆虫细胞中的表达及活性鉴定
- Author:
Zhefeng MENG
;
Ying PENG
;
Bo SHEN
;
Jianxin L
- Publication Type:Journal Article
- Keywords:
EGF-IL-18 fusion proteins;
Insect cells;
Targeting therapy
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To express and purify a new fusion protein harboring human epidermal growth factor receptor(EGFR) binding domain and Interleukin-18(IL-18), as well as preliminary assay biological activity of recombinant proteins.Methods:Fusion protein was expressed in insect cells Spodoptera frugiperda cell (Sf9) by using Bac-to-Bac system, and an abbreviation purification procedure was used to purify fusion protein. IFN-?induction assay and EGF Receptor competitive test was used to determine fusion protein's biological activity.Results:SDS-PAGE and western blot assay showed that purified EGF-IL-18 fusion protein had high purity in 20 kD as expected and had the same antigenic specificity as human IL-18. IFN-?induction assay and EGF Receptor competitive test showed that fusion protein induced production of IFN-?in human PBMC and bound with tumor cells.Conclusion:EGF-IL-18 fusion protein has been successfully expressed and purified in insect cells and shows potential to apply in targeting therapy for tumor.
