Effect of crystallines on survival and growth of rat retinal ganglion cells in vitro
- VernacularTitle:晶体蛋白对离体培养的大鼠视网膜神经节细胞的作用
- Author:
Yanhua WANG
;
Yi WANG
- Publication Type:Journal Article
- Keywords:
Wounds and injuries;
Crystallins;
Retinal ganglion cells;
Cell culture
- From:
Chinese Journal of Trauma
1991;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of crystallins on survival and growth of rat retinal ganglion cells (RGCs) in vitro and study elementarily the exact substance of the injured lens promoting RGCs survival and axonal regeneration. Methods RGCs in Long Evans rats were cultured in DMEM, crystallins and crystallins activated macrophage-conditioned media. The growth regularity and survival time of RGCs in vitro were observed under phase-contrast microscope. The number of RGCs with processes, length of the longest processes and cells activity were measured when RGCs were cultured for 1, 3 and 5 days respectively. Results (1) RGCs could survive for 12-14 days in DMEM containing crystallins, and the survival time of RGCs was longer significantly than other two groups (P
