The effects of basic fibroblast growth factor, transforming growth factor-?1 and bone morphogenetic protein-2 on the alkaline phosphate and calcification cap acity of rat bone marrow stromal cells
- VernacularTitle:rhTGF-?1、rhBMP-2和rhbFGF单独或者联合应用对BMSC的ALP活性和钙化能力影响
- Author:
Hongwei LIU
;
Cailiang LI
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cell;
Basic fibroblast growth factor;
Transforming growth factor-?1;
Morphogenetic protein-2;
Alkaline phospha te;
Capacity
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of basic fibroblast growt h factor(rhbFGF), transforming growth factor-?1(rhTGF-?1) and bone morphogenet ic protein-2(rhBMP-2) on the alkaline phosphate and calcification capacity of rat bone marrow stromal cells(BMSCs) in vitro. Methods: Rat BMSCs were cultured in DMEM with the addition of rhbFGF(1 ?g/L), rhTGF-?1(5 ?g/L) and rhBMP-2(200 ?g/L) alone or in combination.Alkaline phosphoric activ ity of the cells was mesured after 12- and 14-day culture respectively. For th e test of calcification, BMSCs were cultured in minerialization inducing medium with the addtion of above mentioned cytokines, calcification capacity of the cel ls was examined with Vonkossa staining after 10,20 and 30 days culture respectiv ely.Results: rhBMP-2 strengthened the ALP activity, rhbFGF, rh TGF-? 1 alone or in combination inhibited ALP activity, rhTGF-? 1 combined w ith rhBMP-2 and rhbFGF combined with rhBMP-2 inhibited ALP activity;the 3 kind s of growth factor in combination inhibited ALP activity. Vonkossa staining reve aled that rhBMP-2 alone or combined with rhbFGF increased the calcification of BMCs(P
