Effects of enamel matrix proteins on the proliferation and protein synthesis of ectomesenchymal stem cells in vitro
- VernacularTitle:釉基质蛋白对大鼠外胚间充质细胞增殖和蛋白合成影响的体外研究
- Author:
Feng CHAI
;
Yan JIN
;
Yimin ZHAO
- Publication Type:Journal Article
- Keywords:
Enamel matrix proteins;
Ectomesenchymal stem cell s;
Periodontal regeneration;
Proliferation
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of enamel matrix pr ot eins(EMPs) and purified EMPs(EMD) on the proliferation and protein synthesis act ivity of rat ectomesenchymal stem cells in vitro. Methods: C ell culture technique and 3H-leucine label assay were used to measure the pr oliferation and protein synthesis activity of rat ectomesenchymal stem cells exp osed to the EMPs- or EMD-conditioned culture media with different concentratio n. Results: Both EMPs and EMD at 50～200 mg/ml increased the pro l iferation and protein synthesis of the cells in 10-day-culture. EMPs and EMD a t 150 mg/ml showed the strongest effects(P
