The role of neuro-associated autoantibodies in neuropsychiatric systemic lupus erythematosus
- VernacularTitle:神经相关性自身抗体检测在神经精神性狼疮中的意义
- Author:
Xiaofeng SHAO
;
Yongzhe LI
;
Yin SU
;
Wei ZHAO
;
Wei CUI
;
Zhanguo LI
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus.systemic;
Neumpsychiatric;
Neuro-associated anmantibodies;
Indirect immunofluorescence
- From:
Chinese Journal of Rheumatology
2008;12(8):519-523,插一
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the specificities and sensitivities of neuron-assoeiated(Neu-A) autoantibodies in the diagnosis of neuropsychiatric systemic lupus erythematosus(NPSLE)and explore its role in the pathogenesis of NPSLE.Methods The ncuro-associated autoantibodies including anti-neuroblastoma cell,anti-neuroglioma cell,and anti-brain,anti-spinal antibodies were tested using neuroblastoma cell line SK-N-SH.ueuroglioma cell line U251 or cerebrum and spinal meduHa of mice C57BL/10 as substrates respec tively,Serums of 121 SLE patients fincluding 36 NPSLE patients and 85 systemic lupus erythematosus (SLE)patients without CNS involvement),34 rheumatoid arthritis(RA)patients and 34 healthy controls were tested by indirect immunofluorescence(IIF)assay.Cerebrospinal fluid of 24 NPSLE patients and 22 CNS dis eases controls were also tested.Results The positive rates of anti-neuroblastoma cell antibodies,anti-brain and anti-spinal antibodies in serums of SLE patients were 17.4%,25.6%and 29.8%,respectively.Only 2.9%, 5.9%,0 in 34 RA patients and 0,2.9%,0 in health controls were positive(P＜0.05 or P＜0.01).The specificity of the three antibodies in SLE was 98.5%.95.6%and 100%respectively.Anti-neuroblastoma cell antibodies, anti-brain and anti-spinal antibodies were closely associated with SLEDAI score.The NPSLE patients with positive anti-neuroblastoma cell antibodies,anti-brain or anti-spinal antibodies had frequent seizure atlacks, headache,acute confusion state and mood disorders.There was a higher prevalence of anti-neuroblastoma cell.anti-brain and anti-spinal antibodies in NPSLE than those in SLE patients.29.2%anti-brain and anti- spinal antibodies were detected in the eerebrospinal fluid of NPSLE patients.which were significantly higher than those of disease control group(P＜0.05 or P＜0.01).The specificities of these two antibodies jn NPSLE were 100%and 95.5%respectively.Conclusion Anti-neumhlastoma cell antibody.anti-brain and anti spinal antibodies are valuable markers for the diagnosis of SLE.They are closely associated with the disease activity of SLE and neuropsychiatric symptoms.Anti-spinal antibody may be a sensitive and specific marker for the diagnosis of NPSLE while anti-brain and anti-spinal antibodies in the cerebrospinal fluid are more specifie for the diagnosis of NPSLE.
