Cyclosporine A preconditioning to donor kidney reduced apoptosis and expression of NF-?B in renal grafts in vivo
- VernacularTitle:以环孢素A预处理供肾减轻肾脏损伤的实验研究
- Author:
Jiangqiao ZHOU
;
Wei LEI
;
Linglong WANG
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Heat-shock proteins;
NF-kappa B;
Apoptosis;
Cytoprotection
- From:
Chinese Journal of Organ Transplantation
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective mechanism of cyclosporine A to renal autografts in situ.Methods Thirty rabbits were divided into three groups at random (n=10 in each group), subjected to left renal in situ perfusion with normal saline (control group), HC-A solution (HC-A group) and HC-A solution containing CsA (30 ml/L, experimental group) respectively. By ~using self-made in situ cryopreservation device, the left kidney was cryopreserved in situ at 0-4 ℃ for 2 h. Then the right kidney was dissected and the left renal blood flow was opened. At 6th h and 24th h after reperfusion, blood BUN and Cr levels were determined. At 24th h, the left kidneys were ~dissected for the detection of the expression of HSP70 and NF-?B in left renal tissues. The apoptotic rate in left renal tissues was assayed by in situ end-labeling method. Results In the experimental group, the expression level of HSP70 was significantly higher, while the expression of NF-?B and ~apoptotic rate significantly lower than in the other two groups (all P
