Expression of iNOS mRNA prevented cardiac allograft vasculopathy after heart transplantation
- VernacularTitle:诱生型一氧化氮合酶mRNA的表达对心脏移植术后移植物血管病的抑制作用
- Author:
Naishi WU
;
Wangfu ZANG
;
Mingyu ZHANG
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Transplants;
Cardiovascular diseases;
Nitric-oxide synthase
- From:
Chinese Journal of Organ Transplantation
2003;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the prevention of cardiac allograft vasculopathy (CAV) post-transplant through the expression of iNOS mRNA. Methods Rat model of heterotopic heart transplantation (Abdomen) was developed and recipients were injected with Cyclosporin A at abdomen in the meantime of receiving L-arginine (iNOS mRNA group) and inhibitor of iNOS (L-NAME group) according to grouping. Plasma content of NO_3-, the expression of iNOS mRNA in coronary ~artery of transplanted heart and the radio of tunica intima/tunica media thickness (I/M) were assayed after ~operation . Results The plasma content of NO_3-and the expression of iNOS mRNA in coronary ~artery of transplanted heart in iNOS mRNA group were obviously higher than those in control group and L-NAME group during 2 to 4 weeks after operation. The ratio of I/M in iNOS mRNA group was ~lower than that in control group and L-NAME group 4 weeks after operation. Conclusion The ~expression of iNOS can promote the synthesis of NO and prevent CAV development.
