Changes of splenic T-lymphocyte subsets in rats with acute pancreatitis
- VernacularTitle:大鼠急性胰腺炎脾脏T淋巴细胞亚群的改变
- Author:
Shengji YANG
;
Yon SUN
- Publication Type:Journal Article
- Keywords:
Spleen?Pancreatitis?T-lymphocyte subsets
- From:
Chinese Journal of Current Advances in General Surgery
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To examine T-lymphocyte subsets of peripheral blood and splenic cells in rat with acute pancreatitis(AP),to investigate the changes of immune functional status in the development of acute pancreatitis.Methods: AP model was developed in adult male rats by retrograde injection of 4% sodium taurocholate in the pancreatic duct.T-lymphocyte subsets( CD3,CD4,CD8) of peripheral blood and splenic cells was measured by Flow Cytometer.Results: There was no singificant change in the stage of MAP.In the stage of SAP,the levels of CD3 and CD4 and CD4/CD8 of peripheral blood were decreased,the decrease of T-lymphocyte subsets in splenic cells was more obvious than those in peripheral blood.Conclusion:Spleen shows a negative immunologic function in the stage of SAP and promote the development of AP.
